In microscopy, the depth of field refers to the range of distance that runs parallel to the optical axis where the specimen can move and still be viewed without negatively affecting the clarity of the object under observation. The depth of field determines the vertical extent of the plane of focus, which is typically measured in microns.Continue Reading
In various scientific fields, a microscope is an important tool in viewing and examining objects that are too small to be seen by the naked eye. One of the basic components of a microscope is an eyepiece or ocular lens. A nosepiece, which has several objective lenses is located below the eyepiece. The objective lenses are calibrated into different levels of magnification. Depending on what magnification the specimen is to be viewed, the nosepiece can be easily rotated and the objective lens set into place. Other parts include a mechanical stage, substage condenser, iris diaphragm, substage illuminator, a rheostat and two knobs that are adjusted to focus the sample specimen.
When viewing an object, the overall magnification is calculated by multiplying the value of the ocular lenses with the value of the objective lenses. The total magnification is indirectly proportional to the depth of field. As magnification increases, the axial resolving power of an objective, which is the depth of field, is reduced.Learn more about Chem Lab
In the physical sense, breadth is defined as the measure of the second-largest dimension of an object or its width, whereas depth is generally the distance from the top to bottom or from the front to back of an object. Breadth and depth are also used to explain how a person understands the world. People can have breadth or depth knowledge about particular subjects, notes the University of Guelph.Full Answer >
Light microscope disadvantages include limited resolution, lower magnification and poorer surface view of the specimen. Compound light microscope require the user to adjust to optical inversion, meaning the specimen will appear to be displaced in the opposite direction it is moved.Full Answer >
A diaphragm on a microscope is the piece that enables the user to adjust the amount of light that is focused under the specimen being observed. A diaphragm is typically found on higher-power microscopes versus less expensive or toy models.Full Answer >
Calculating electric field intensity requires the knowledge about the current charge level of the object that is making the electric field and the distance between that charged object and the measuring tool. Electric field intensity (or strength) is a vector quantity, which means it has direction as well as magnitude. The magnitude of this intensity comes from the way it is measured.Full Answer >