According to the United States Nuclear Regulatory Commission, the acceptable temperature range for a drug test urine sample is from 90 to 100 degrees Fahrenheit. Samples should be at least 30 milliliters, but samples above 15 milliliters can be tested. Temperature must be measured within 4 minutes of urination.
The NRC also requires the assessment of urine color, clarity and signs of contamination to determine if the sample is legitimate. All observations are recorded by the collector. If the collector suspects a possibly illegitimate sample, the sample must be reported to the Health and Human Services Certified laboratory at once.