Giemsa stain is a staining reagent that was developed in the early 20th century by Gustav Giemsa to aid in cellular microscopy. Different cells and parts of cells stain slightly different colors, allowing appropriate diagnoses to be made.
PRINCIPLE OF GIEMSA STAINING. Giemsa stain is commonly used when there is a need to examine the Blood smear for the Parasites but is a good stain for routine examination of blood smear and used to differentiate nuclear and cytoplasmic morphology of the various cells of the blood like Platelets, RBCs, WBCs as well as the parasites.
Wright and Giemsa stains are Romanowsky stains used to stain peripheral blood and bone marrow smears. The most important components of these stains are oxidized methylene blue, azure B and eosin Y dyes. The eosin Y dye stains the cytoplasm of cells an orange to pink color. The methylene blue and azure B dyes stain the nucleus varying shades of ...
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
Light microscopic examination of a peripheral blood smear stained with Wright or Giemsa stain is a rapid, but insensitive, method for diagnosis of ehrlichiosis. The presence of characteristic dark blue or purple staining cytoplasmic inclusions containing bacteria known as morulae in monocytes ( E. chaffeensis ) ( Fig. 109.3 ) or neutrophils ( E ...
Wright’s stain is a type of Romanowsky stain, which is commonly used in hematology laboratory for the routine staining of peripheral blood smears. It is also used for staining bone marrow aspirates, urine samples and to demonstrate malarial parasites in blood smears. Wright’s stain is named for James Homer Wright, who devised the stain in 1902 based on a modification of Romanowsky stain.
Giemsa stain also is used to stain Histoplasma capsulatum, Pneumocystis jiroveci, Klebsiella granulomatis, Penicillium marneffei and occasionally bacterial capsules. This stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations. It is commonly used for G-banding (Giemsa-Banding) Principle of Giemsa Stain ...
3. Place slide on staining rack, cover with Wright stain, 5 minutes. 4. Do not drain off stain, add an equal amount of distilled water until a metallic sheen appears. Leave for 5 minutes. 5. Place slides directly into the Giemsa solution, for 45 minutes, room temperature. 6. Differentiate and dehydrate in the following: acetic water 3 dips ...
Romanowsky staining, also known as Romanowsky–Giemsa staining, is a prototypical staining technique that was the forerunner of several distinct but similar stains widely used in hematology (the study of blood) and cytopathology (the study of diseased cells). Romanowsky-type stains are used to differentiate cells for microscopic examination in pathological specimens, especially blood and bone ...
stain because Wright’s-Giemsa Stain is formed when Wright’s stain is combined with Giemsa. Therefore, it can be used in the study of pathogenic bacteria attached to human cells. Here, the human cells and the bacterial cells are stained differentially and purple and pink colors are observed respectively.