The dip method is a quick staining method that uses a modified Wright -Giemsa stain buffered in methanol at pH 6.8. Slides are immersed in the stain in a coplin jar for a user -determined length of time. It is important that the Wright-Giemsa stain be kept tightly sealed in coplin jar when not in use and be replaced when water artifact
Staining and staining problems There is 2 main types of stains that is used most commonly. They are the Romanowsky and Papanicolaou(and it's derivatives). Romanowsky-type stain are more rewarding and practical, and readily available in practice situations. Example of such stains are Wright's stain, Giemsa stain, Diff-Quik.
The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify ...
Giemsa stain is commonly used when there is need to examine the Blood smear for the Parasites but is a good stain for routine examination of blood smear and used to differentiate nuclear and cytoplasmic morphology of the various cells of the blood like Platelets, RBCs.... Giemsa Staining Technique....
The Wright-Giemsa stain is a modified Romanowsky stain composed of a combination of basic dyes, viz., methylene blue and its oxidative products, azure A and azure B, and an acidic dye, eosin. The stain is used routinely in hematology laboratories to stain peripheral blood and bone marrow aspirate smears.
Wright-Giemsa Stain, Modified : When blood films are stained using Wright-Giemsa Stain, the white blood cell nucleus and cytoplasm take on the characteristic blue or pink coloration. The combination of purified eosin and thiazine dyes in the product eliminates inconsistent staining and yields reproducible chromogenic responses.
Staining the Blood Smear After the smear has been produced properly, it is ready for Wright's staining. The mechanism of Wright's staining is a proc ess in which both chemical and physical factors playa role. During the staining proc ess, the stain in methanol is directly applied to the smear. The alcoholic solution tends to
Wright staining is used to differentiate blood cells which consist of a mixture of eosin and methylene blue dyes. Giemsa staining is utilized during the staining of bacterial cells as well as human cells and could be combined with Wright stain to develop Giemsa Wright stain. This is the key difference between Giemsa stain and Wright stain. CONTENTS
Stain Paks. The Stain Pak consists of one bottle each of stain, buffer, and rinse solutions, and is designed for easy installation and removal from the instrument. NOTE Only Hematek Stain Paks should be used with the Hematek Slide Stainer. Use of other stain packs or solutions may void the warranty.
1. Place approximately 50 ml Wright-Giemsa Stain in a Coplin jar. 2. Fill another Coplin jar with water or phosphate buffer. 3. Place thoroughly dried blood film,feather edge DOWN, in Wright-Giemsa Stain for approximately 30 seconds. NOTE: Rapid dipping for 5-10 seconds may reduce water artifacts on films that are not thoroughly dried. 4.