Giemsa stain is a staining reagent that was developed in the early 20th century by Gustav Giemsa to aid in cellular microscopy. Different cells and parts of cells stain slightly different colors, allowing appropriate diagnoses to be made.
The Procedure of Giemsa staining varies as per the purpose of staining that means whether the staining is done for the examination of Blood cells or to find the Parasites in the blood smear and accordingly the Blood smears are prepared as Thin Blood films or Thick blood films.
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
The Thermo Scientific™Richard-Allan Scientific Wright-Giemsa Stain Solution is a mixture of several thiazin dyes in a methanol solvent. Ionic and nonionic forces are involved in the binding of these dyes. The staining solution has anionic and cationic properties.
Staining: Place 1.0ml of the Wright-Giemsa Stain (#26149-01) upon the smear, in sufficient quantity to cover the entire surface, for 3-4 minutes. Add 2.0ml distilled water or Phosphate Buffer, pH 6.5 (#26149-02) and let stand twice as long as in step 1.
Wright’s stain is a polychromatic stain consisting of a mixture of Eosin and Methylene blue. As the Wright stain is methanol based, it doesn’t require a fixation step prior to staining. However, fixation helps to reduce water artefact that can occur on humid days or with aged stain. Methanol fixes the cells to the slide.
Wright Giemsa Stains Wright and Giemsa stains are Romanowsky stains used to stain peripheral blood and bone marrow smears. The most important components of these stains are oxidized methylene blue, azure B and eosin Y dyes. The eosin Y dye stains the cytoplasm of cells an orange to pink color.
Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope.
Prepare fresh working Giemsa stain in a staining jar, according to the directions above. (The 40 ml fills adequately a standing Coplin jar; for other size jars, adapt volume but do not change proportions). Pour 40 ml of working Giemsa buffer into a second staining jar. Add 2 drops of Triton X-100.
Giemsa stain is a differential stain and contains a mixture of Azure, Methylene blue, and Eosin dye. It is specific for the phosphate groups of DNA and attaches itself to where there are high amounts of adenine-thymine bonding. Azure and eosin are acidic dye which variably stains the basic components of the cells like the cytoplasm, granules etc.