Polymerase chain reaction (PCR) is a method widely used in molecular biology to make many copies of a specific DNA segment. Using PCR, copies of DNA sequences are exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.
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Define polymerase chain reaction. polymerase chain reaction synonyms, polymerase chain reaction pronunciation, polymerase chain reaction translation, English dictionary definition of polymerase chain reaction. ... The polymerase chain reaction, or PCR, found its way into many areas in biology very soon after its invention by the American Kary ...
polymerase [pah-lim´er-ās] an enzyme that catalyzes polymerization. polymerase chain reaction a rapid technique for in vitro amplification of specific DNA or RNA sequences, allowing small quantities of short sequences to be analyzed without cloning. pol·ym·er·ase chain re·ac·tion (PCR), an enzymatic method for the repeated copying of the two ...
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Polymerase chain reaction definition is - an in vitro technique for rapidly synthesizing large quantities of a given DNA segment that involves separating the DNA into its two complementary strands, using DNA polymerase to synthesize two-stranded DNA from each single strand, and repeating the process —abbreviation PCR.
RT-PCR (Reverse transcriptase-polymerase chain reaction) is a highly sensitive technique for the detection and quantitation of mRNA (messenger RNA). The technique consists of two parts: The synthesis of cDNA (complementary DNA) from RNA by reverse transcription (RT) and ; The amplification of a specific cDNA by the polymerase chain reaction (PCR).
The polymerase chain reaction (PCR) was originally developed in 1983 by the American biochemist Kary Mullis. He was awarded the Nobel Prize in Chemistry in 1993 for his pioneering work. PCR is used in molecular biology to make many copies of (amplify) small sections of DNA or a gene.
Definition: An in-vitro DNA amplification technique that allows synthesizing millions of copies of the gene or DNA of interest from a single copy • It is called “polymerase” because the only enzyme used in this reaction is DNA polymerase.
The polymerase chain reaction enables investigators to obtain the large quantities of DNA that are required for various experiments and procedures in molecular biology, forensic analysis, evolutionary biology, and medical diagnostics.