Giemsa stain is a staining reagent that was developed in the early 20th century by Gustav Giemsa to aid in cellular microscopy. Different cells and parts of cells stain slightly different colors, allowing appropriate diagnoses to be made.
Malaria is caused by a bite from a parasite-infected mosquito. According to WebMD, five species of parasites infect people. They are mainly found in the tropics and subtropics near the equator of Africa and South America. The bite of an infected P. falciparum mosquito can be life-threatening.
If untreated or treated improperly, malaria can last the lifetime of the infected individual. Malaria is curable if treated properly, states the CDC, but treatment time varies depending on health factors of the infected, where the person was infected, and the amount of time that the person has been
Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution. It was primarily designed for the demonstration of malarial parasites in blood smears, but it is also employed in histology for routine examination of blood smear.
The Giemsa stain is one of the best stains for malaria and other blood parasites and also satisfactory as a routine bloodstain to stain the Peripheral blood smear for the examinations of blood film under the microscope.
Laboratory diagnosis of malaria Staining for malaria parasites . Staining Blood Smears . Stain only one set of smears, and leave the duplicates unstained. The latter will prove useful if a problem oc-curs during the staining process and/or if you wish later to send the smears to a reference laboratory. Giemsa stain
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
Tag: giemsa stain Microscopic Tests. Diagnosis of malaria involves identification of malaria parasite or its antigens/products in the blood of the patient. Although this seems simple, the efficacy of the diagnosis is subject to many factors. The different forms of the four malaria species; the different stages of erythrocytic schizogony; the ...
Giemsa stain is also used to visualize chromosomes.This is particularly relevant for detection of Cytomegalovirus infection, where the classical finding would be an "owl-eye" viral inclusion.. Giemsa stains the fungus Histoplasma, Chlamydia bacteria, and can be used to identify mast cells.. Generation. Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.
The Giemsa stain is one of the best stains for malaria and other blood parasites and also satisfactory as a routine blood stain to stain the Peripheral blood smear for the examinations of blood film under the microscope.