I'm currently attempting to use a pair of m13 tailed primers purchased from thermo Hs00836745_CE (google this) to sequence a BCOR mutation with amplicon ...
M13 Tailed Primer Protocol. Primer Design. “M13” labeled sequences: FAM = TTT CCC AGT CAC GAC GTT G. NED = TAA AAC GAC GGC CAG TGC.
We provide great primer design tools and an attractive price for primers up to 45 bases long. The main advantage of using an M13 tailed primer or universal ...
Apr 17, 2001 ... M13-Tailed Primers Im- prove the Readability and. Usability of Microsatellite. Analyses Performed with. Two Different Allele-. Sizing Methods.
Using the M13-tailed primer method, we identified 45 novel polymorphic microsatellite markers from microsatellite-enriched Cx. tarsalis genomic libraries.
gests the bacterially methylated tem- plate plasmid DNA at 5′-GATC-3′ se- quences and leaves newly synthesized, unmethylated DNA intact. Two micro-.
Oct 10, 2016 ... Non-specific tail sequences are often added to the 5'-terminus of primers to improve the robustness and overall performance of diagnostic ...
PCR primers were selected using the program PCR-Overlap. Gene specific primers were "5' tailed" with an M13 universal sequence to make them compatible ...
Apr 11, 2012 ... In the first PCR step, all forward primers are concatenated with a universal M13 tail at their 5′ end, while reverse primers are 5′‐appended ...
Tailed Primers. In this approach, one of the unlabeled STR primers is synthesized with an M13 forward or reverse primer sequence on the 5'-end.