Web Results

www.researchgate.net/post/Why_use_M13F_tailed_primers_for_PCR_and_sequencing...

Having said that, I would recommend that the sequencing primer is just the M13F sequence, not the full primer used to amplify the locus (i.e., don't use M13F tailed primers for PCR and sequencing ...

www.vgl.ucdavis.edu/dogmap/protocols/M13 Tailed Primer Protocol.doc

Primer Mixes. Labeled primer mix stock. 52 µls of 500 uM M13, dye-labeled oligos (Applied Biosystems) added to 50 mls of 10 mM Tris. each of the three labeled M13 primers at 0.52 uM in primer mix stock. Add the following to 2990 uls of M13 primer mix stock: 7.7 µls of each 100 uM Forward (usually a 40-mer; contains the M13 leader sequence)

www.ncbi.nlm.nih.gov/pmc/articles/PMC3243645

In this study, the use of fluorescently-labeled M13-tailed primers allowed us to eliminate the acrylamide step and economically screen all primer sets directly on the capillary sequencer. At a fraction of the cost, we were able to generate almost 4 times as many usable markers in approximately one third the time compared to our previous study.

www.thermofisher.com/us/en/home/life-science/sequencing/sanger-sequencing/...

The most common tail is the M13 sequence because it was initially used for sequencing clones constructed in the single-stranded bacteriophage M13. Potential disadvantages of using tailed PCR primers are the greater challenge in designing primers with a tail and the need for higher-quality oligonucleotides due to the increase in primer length ...

bitesizebio.com/13562/some-sanger-sequencing-tips-and-tricks

M13 tailed PCR primers: M13 is the most commonly used multiple-cloning priming site. Nearly all Sanger sequencing providers carry M13 forward and reverse primers ready for you to use free of charge. Nearly all Sanger sequencing providers carry M13 forward and reverse primers ready for you to use free of charge.

www.vgl.ucdavis.edu/dogset/help.jsp

Primer Mixes Labeled primer mix stock. 52 µls of 500 uM M13, dye-labeled oligos (Applied Biosystems, see also M13 Tailed Primers) added to 50 mls of 10 mM Tris buffer; each of the three labeled M13 primers at 0.52 uM in primer mix stock

www.researchgate.net/publication/11876801_M13-Tailed_Primers_Improve_the...

PDF | On Aug 1, 2001, Isabelle Boutin-Ganache and others published M13-Tailed Primers Improve the Readability and Usability of Microsatellite Analyses Performed with Two Different Allele- Sizing ...

www.academia.edu/23693606/M13-tailed_primers_improve_the_readability_and...

M13-tailed primers, which can be obtained inexpensively either from vendors or by custom syn- M13-Tailed Primers Im- thesis, have previously been shown to prove the Readability and amplify infrared dye-labeled alleles that can be analyzed on an IR2 Auto- Usability of Microsatellite mated DNA sequencer (LI-COR, Lin- Analyses Performed with coln ...

journals.plos.org/plosone/article?id=10.1371/journal.pone.0164463

Non-specific tail sequences are often added to the 5’-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in vitro and in silico analysis of the enhancing effect of primer tailing on 2 well-established ...

www.ncbi.nlm.nih.gov/pmc/articles/PMC4103466

An efficient technique for primer development and application that integrates fluorescent labeling and multiplex ... a forward tailed primer that is tagged with a specific DNA sequence (the “tail”) attached to the 5 ... M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele ...