Web Results

www.researchgate.net/post/Why_use_M13F_tailed_primers_for...

While your PCR products should be tailed with both M13 forward and M13 reverse primers -- to allow you to sequence each product in both directions, you must be careful to use the same sequencing ...

www.vgl.ucdavis.edu/dogmap/protocols/M13 Tailed Primer Protocol.doc

each of the three labeled M13 primers at 0.52 uM in primer mix stock. Add the following to 2990 uls of M13 primer mix stock: 7.7 µls of each 100 uM Forward (usually a 40-mer; contains the M13 leader sequence) x 3 (multiplex 3-deep) ... M13 Tailed Primer Protocol ...

www.ncbi.nlm.nih.gov/pmc/articles/PMC3243645

In this study, the use of fluorescently-labeled M13-tailed primers allowed us to eliminate the acrylamide step and economically screen all primer sets directly on the capillary sequencer. At a fraction of the cost, we were able to generate almost 4 times as many usable markers in approximately one third the time compared to our previous study.

bitesizebio.com/13562/some-sanger-sequencing-tips-and-tricks

M13 tailed PCR primers: M13 is the most commonly used multiple-cloning priming site. Nearly all Sanger sequencing providers carry M13 forward and reverse primers ready for you to use free of charge. Nearly all Sanger sequencing providers carry M13 forward and reverse primers ready for you to use free of charge.

www.thermofisher.com/.../pcr-sanger-sequencing.html

The most common tail is the M13 sequence because it was initially used for sequencing clones constructed in the single-stranded bacteriophage M13. Potential disadvantages of using tailed PCR primers are the greater challenge in designing primers with a tail and the need for higher-quality oligonucleotides due to the increase in primer length ...

www.promega.com/.../puc_m13-sequencing-primers

pUC/M13 Sequencing Primers. Reverse 17mer/2µg. $ 91.00. Your price: Log in. Change Configuration. The pUC/M13 Primers are used to sequence inserts cloned into the M13 vectors and pUC plasmids developed by Messing. The primers are purified by gel electrophoresis or HPLC and supplied in sterile water.

www.researchgate.net/publication/6434867_5_'-Tailed...

In most cases (7 out of 8), the products obtained with the tailed primers were found to have less than half the number of sequencing errors than those obtained by the nontailed primers.

www.vgl.ucdavis.edu/dogset/help.jsp

Primer Mixes Labeled primer mix stock. 52 µls of 500 uM M13, dye-labeled oligos (Applied Biosystems, see also M13 Tailed Primers) added to 50 mls of 10 mM Tris buffer; each of the three labeled M13 primers at 0.52 uM in primer mix stock

www.ncbi.nlm.nih.gov/pmc/articles/PMC4103466

An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR 1. ... added to the same PCR reaction: (1) a forward tailed primer that is tagged with a specific DNA ... M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele ...

tools.thermofisher.com/content/sfs/brochures/primer-designer-tool...

Primer Designer™ tool makes primer selection for re-sequencing easy NGS users may want to verify the finding of a SNP or other variant in the human exome if an actionable decision depends on the accuracy of the result. To that end, the locus of interest has to be re-analyzed ... • Non-tailed or M13-tailed primers can be used