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www.answers.com/Q/Why_does_pH_affect_fluorescence_spectroscopy

Fluorescence spectroscopy is a type of spectroscopy that analyzes fluorescence from a provided sample. This uses a beam of light, often an ultraviolet light which then causes absorption ...

micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorescenceintro.html

Solvent Effects on Fluorescence Emission. A variety of environmental factors affect fluorescence emission, including interactions between the fluorophore and surrounding solvent molecules (dictated by solvent polarity), other dissolved inorganic and organic compounds, temperature, pH, and the localized concentration of the fluorescent species.

pubs.acs.org/doi/abs/10.1021/ac60291a034

pH Effects on fluorescence of umbelliferone. David W. Fink, and Walter R. Koehler. ... Quantitative treatment of the effect of solvent on the electronic absorption and fluorescence spectra of substituted coumarins: Evaluation of the first excited singlet-state dipole moments.

olympus.magnet.fsu.edu/primer/java/jablonski/solventeffects/index.html

Solvent Effects on Fluorescence Emission. A variety of environmental factors affect fluorescence emission, including interactions between the fluorophore and surrounding solvent molecules (dictated by solvent polarity), other dissolved inorganic and organic compounds, temperature, pH, and the localized concentration of the fluorescent species.

www.chem.uci.edu/~dmitryf/manuals/Fundamentals/Fluorescence Spectroscopy.pdf

An Introduction to Fluorescence Spectroscopy 7 Fluorescence At room temperature most molecules occupy the lowest vibrational level of the ground electronic state, and on absorption of light they are elevated to produce excited states. The simplified diagram below shows absorption by molecules to

www.sciencedirect.com/science/article/pii/0048969789901186

Concentration- and pH-dependence effects have been studied on the fluorescence spectra measured in the emission, excitation and synchronous-scan excitation modes of five humic acid-like polymers synthesized by soil fungi in culture media.

www.sciencedirect.com/science/article/pii/0022231375900034

The dotted lines indicate that the occurrence of the different species does not correspond to the true equilibrium at these pH values. 390 M.M. Martin, L. Lindqvist / pH dependence of fluorescein fluorescence that in HPF internal conversion is the main deactivation channel; one may assume that this applies also in the case of the neutral ...

www.thermofisher.com/.../introduction-to-fluorescence-techniques.html

Fluorescence is the result of a three-stage process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes (Figure 1).A fluorescent probe is a fluorophore designed to respond to a specific stimulus or to localize within a specific region of a biological specimen.

onlinelibrary.wiley.com/doi/abs/10.1111/j.1751-1097.1983.tb03869.x

Abstract— The influence of pH in the range 1.0 to 13.0 on the structure of sanguinarine has been investigated by spectrophotometric and spectrofluorimetric measurements. The data on absorption maxima, molar extinction coefficient, fluorescence emission maxima, relative fluorescence intensity and fluorescence quantum yield of the sanguinarine under various pH are presented.

www.chem.utoronto.ca/coursenotes/CHM317/pdfs/CHM317Fluor.pdf

As part of the characterisation, you will study the effect of pH on quinine fluorescence. After performing this experiment, you should: – Understand the connection between structure, pH, and fluorescence – Understand the difference between excitation and emission spectra – Be aware of the high concentration limits in fluorescence measurement