⇒ Diluting the Giemsa Stain for Thin Blood smear: For staining the thin blood smear the Giemsa stain is used in 1:20. To make 1:20 dilution of Giemsa stain add 2 ml of stock solution of Giemsa stain to 40 ml of phosphate buffer solution in a clean Coplin jar. You can also use the Distilled water instead of buffer but the results may vary.
Giemsa stain is also used to visualize chromosomes.This is particularly relevant for detection of Cytomegalovirus infection, where the classical finding would be an "owl-eye" viral inclusion.. Giemsa stains the fungus Histoplasma, Chlamydia bacteria, and can be used to identify mast cells.. Generation. Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.
Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution. It was primarily designed for the demonstration of malarial parasites in blood smears, but it is also employed in histology for routine examination of blood smear.
Wright and Giemsa stains are Romanowsky stains used to stain peripheral blood and bone marrow smears. The most important components of these stains are oxidized methylene blue, azure B and eosin Y dyes. The eosin Y dye stains the cytoplasm of cells an orange to pink color.
Romanowsky stains also stain granules differentially. The most commonly used Romanowski stains are giemsa stain using giemsa dye, wright stain,may grunwald stain and Jenner’s stain but of all these stains,Leishman and Wright are widely used in routine staining. However, the results are inferior as compared to Giemsa and Jenner stain.
PREPARATION OF GIEMSA STAIN IN LABORATORY. Giemsa stain is a differential stain that is used to stain the various components of the cells (blood or Aspirated Fluid) and it can be used to study the adherence of pathogenic bacteria to the human cells. Giemsa stain is a mixture of Azure, Methylene blue, and Eosin dye.
Wright-Giemsa Stain Method EMS Catalog #: 26149-Series Fixation: Streak thin (approx. one cell thick) smears across a sterile slide by means of a second slide or cover glass. Air-dry quickly. Staining: Place 1.0ml of the Wright-Giemsa Stain upon the smear, in sufficient quantity to cover the entire surface, for 3-4 minutes.
stain, resulting in poor staining when the equipment is next used. Caution During staining with Giemsa stain (3% or 10% stain working solution), the surface becomes covered with a metallic green scum. Avoid getting it onto blood films during rinsing, as it can impair examination.
Quality Control: Always check new batches of stain and reagents for correct staining reactions using a smear containing known Gram-positive and Gram-negative organisms. Variations in Gram Staining Results Various factors influence the results of Gram staining. Sometime the result might be entirely different than you have anticipated.
The Giemsa Blood Smear Test is a test to detect blood parasites. It is used to diagnose diseases, such as malaria, which are caused by parasitic infections The collected blood sample is smeared onto a glass slide and stained with Giemsa stain.