Giemsa stain is also used to visualize chromosomes.This is particularly relevant for detection of Cytomegalovirus infection, where the classical finding would be an "owl-eye" viral inclusion.. Giemsa stains the fungus Histoplasma, Chlamydia bacteria, and can be used to identify mast cells.. Generation. Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.
stain [stān] 1. a substance used to impart color to tissues or cells, to facilitate microscopic study and identification. 2. an area of discoloration of the skin. acid-fast stain a staining procedure for demonstrating acid-fast microorganisms. differential stain one that facilitates differentiation of various elements in a specimen. endogenous stain an ...
Giemsa stain is called a differential stain because it produces different colors depending on what it bonds to, such as cytoplasm or DNA. The formula for Giemsa stain has been adjusted over time to improve the stability of the dyes and the colors that result. Current standard mixtures include methylene blue, eosin, and sometimes azure B.
The blood films stained with Giemsa or Romanowsky stain show ringlike intraerythrocytic parasites. Morphology is variable, and ring, rod, and ameboid forms of Babesia parasites may be seen (Fig. 158.16). 276 Occasionally, multiple intraerythrocytic forms can be seen, linked to form a tetrad or, colloquially, a “Maltese cross.” However, at low parasitemia, Babesia parasites may easily be ...
Giemsa stain is a staining reagent that was developed in the early 20th century by Gustav Giemsa to aid in cellular microscopy. Different cells and parts of cells stain slightly different colors, allowing appropriate diagnoses to be made.
Giemsa stain definition is - a stain consisting of eosin and a blue dye and used chiefly in the differential staining of blood films —called also Giemsa, Giemsa's stain. How to use Giemsa stain in a sentence.
A brief Description of Giemsa Stain…. Giemsa stain is a mixture of Azure, Methylene blue, and Eosin dye. Giemsa stain is a differential stain that is used to variably stain the various components of the cells and it can be used to study the adherence of pathogenic bacteria to the human cells.
Giemsa solution contains methylene blue, Azure B and eosin and the stain is prepared commercially with the use of Giemsa powder. The stability of the stain depends on methylene azure and its mixture along with methylene blue which forms an eosinate.
Wright and Giemsa stains are Romanowsky stains used to stain peripheral blood and bone marrow smears. The most important components of these stains are oxidized methylene blue, azure B and eosin Y dyes. The eosin Y dye stains the cytoplasm of cells an orange to pink color.
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