molecular hybridization in molecular biology, formation of a partially or wholly complementary nucleic acid duplex by association of single strands, usually between DNA and RNA strands or previously unassociated DNA strands, but also between RNA strands; used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands.
In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid or ribonucleic acid molecules anneal to complementary DNA or RNA. Though a double-stranded DNA sequence is generally stable under physiological conditions, changing these conditions in the laboratory (generally by raising the surrounding temperature) will cause the molecules to ...
In chemistry, orbital hybridisation (or hybridization) is the concept of mixing atomic orbitals into new hybrid orbitals (with different energies, shapes, etc., than the component atomic orbitals) suitable for the pairing of electrons to form chemical bonds in valence bond theory.Hybrid orbitals are very useful in the explanation of molecular geometry and atomic bonding properties and are ...
How to Determine the Hybridization of a Molecular Compound. Determining the hybridization can be difficult. This wikiHow will help you determine the molecular geometry and the hybridization of the molecular compound. First you must draw...
Hybridization time is a significant challenge for an approach that adopts a sequential hybridization and imaging approach to readout barcodes. This challenge arises from the observation that hybridization of DNA oligonucleotides to cellular RNA is a slow process, typically requiring 6–24 h for efficient labeling (Shaffer, Wu, Levesque, & Raj, 2013) because sequences on the cellular RNAs ...
Molecular Hybridization. Molecular hybridization is the association between strands of DNA and RNA whose kinetics are governed by the degree of intra- and interstrand relatedness, solvent type, solvent ionic strength, temperature, and time. From: Molecular Methods for Virus Detection, 1995.
This organic chemistry video tutorial explains the hybridization of atomic orbitals. It discusses how to determine the number of sigma and pi bonds in a molecule as well determining if a carbon is ...
Molecular hybridization of nucleic acids 1. Chapter 4 Molecular Hybridization of Nucleic acids 2. • Molecular hybridization of nucleic acids is the process in which two single-stranded nucleic acid molecules with complementary base sequences form a double-stranded nucleic acid molecule.
Start studying Genetics Test 2 Mastering. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Search. ... the case of polyploidy when the chromosomes originate from the hybridization of two different species, usually closely related. ... What is the chemical basis of molecular hybridization?
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