According to Tusculum College, homologues, or homologous chromosomes, separate during anaphase I of meiosis I. BiologyCorner.com states, "the spindle fibers move homologous chromosomes to opposite sides." During metaphase II, the chromosomes line up again but not in homologous pairs.
The separation of homologous chromosomes allows for each homologue to reach each gamete. This enables each daughter cell to have only 23 chromosomes, which is one from each homologous pair. Sister chromatids remain intact during meiosis I but split during meiosis II. After crossing over occurs, the chromatids separate during anaphase II and line up along the equator during metaphase I of meiosis I.