Objects appear upside down when viewed using a microscope due to the fact that many microscopes use multiple lenses; this causes rays of light to cross, which results in an inverted object. A microscope that utilizes multiple lenses is known as a compound microscope. When using a compound microscope, users must remember that the slide is being viewed upside down and back-to-front.
When the scientist moves the slide to the left, it will appear to move to the right when viewed using the microscope.
When novice users practice utilizing the microscope, it is best to keep both eyes open rather than shutting the eye that is not peering through the lens. This practice helps prevent eye strain.