The mechanism of staining in fungi by lactophenol cotton blue is the use of lactophenol as a cleaning agent, which means that it is able to kill the organism. Then, lactic acid preserves the structure of the fungus. Cotton blue is able to stain the cytoplasm with a certain hue of light blue color so that it is possible for scientists to observe the slide that contains the fungal cells.
Staining a sample of a cell is the process of coloring or distinguishing certain parts of the cell from one another visually. This allows scientists to differentiate the parts of the cell and observe them more clearly. Staining eukaryotic organisms can be vital to human understanding of certain biological processes and phenomena. Understanding certain types of fungi can be helpful in the development of antibiotics and other products and is even used for fermentation or prevention of diseases in agricultural industries.
When using the lactophenol cotton blue staining technique, a scientist must take a clean slide and add a drop of lactophenol cotton blue solution onto it. Then, a sterilized needle can transfer a mat onto this fluid before it is covered with another slip. This allows the lactophenol to clean and kill the organism, and the lactic acid maintains its shape. The cell can then be dyed and observed.