Why Is Heat Necessary in Spore Staining?

By Staff WriterLast Updated Apr 4, 2020 12:20:08 AM ET

Heat is necessary when staining spores because the application of heat disrupts vegetative cells and causes green malachite to be rinsed from them, which then allows the counterstain into the cells. This is a required step in certain spores that are resistant to traditional methods of spore staining.