Gas liquid chromatography involves the vaporization and injection of a sample onto the top of the chromatographer's column. After that, the sample goes through the column as a result of the flow of mobile gases, while the column itself has a stationary liquid phase that adsorbs onto an inert solid surface. At this point, several different detectors can be used, yielding different sorts of selectivity.Continue Reading
It is important for the carrier gas to be inert chemically, and gases that are frequently used include helium, nitrogen, carbon dioxide and argon; the specific choice generally depends on the type of detector in use.
The sample should enter the column as a vapor "plug," as a slower injection of larger samples weakens resolution and broadens the band. Most commonly, a microsyringe injects sample into a vaporizer port through a rubber septum at the column head.
To create precise outcomes, it is important to control temperatures within the column within tenths of a degree, but the optimal temperature varies with the sample's boiling point. A temperature just above the average excess of the sample yields elution time ranging between two or three minutes to half an hour. Samples that have a wide boiling range tend to respond well to temperature programming.Learn more about Chem Lab