An electron microscope has a higher resolution than a light microscope, which results in much finer detail of the specimen being viewed. Although an electron microscope can magnify a specimen up to 2 million times, a light microscope is advantageous when viewing live cells.Continue Reading
Electron microscopy uses an electron beam rather than light to achieve magnification. To do this, electrons pass through a thin piece of the specimen. The wavelengths of an electron beam are much smaller than light, allowing for better resolution. Because of its ability to reach such acute levels of resolution, it is possible to see the shape of a specimen. Although the finite detail is beneficial in many circumstances, the specimen viewed under an electron microscope must be fixed during preparation so that the structure is not disturbed, which means the specimen will not be alive and can only observe its visual characteristics.
Light microscopy functions on the basis of diffraction. To achieve magnification, light either passes through or reflects off of a specimen before it is transmitted through the optical lenses. In certain conditions, live cells and their biological functions, such as food intake and cell division, can be viewed. Viewers can also track proteins and observe cell movement.Learn more about Biology
The first idea for cloning came about in 1938 when Hans Spermann suggested an experiment where one would replace the nucleus of an egg with another cell and grow a new specimen from that. Cloning has now moved on from frogs and rabbits to sheep and dogs.Full Answer >
In light microscopy, lower magnification objective lenses are further from the specimen and survey a larger area, meaning more light enters the microscope, explains How Stuff Works. More magnification means the lens is closer to the stage, which also means less light enters the microscope, making the field of view dimmer.Full Answer >
Wet mounts should ideally have no air bubbles because beginners may have trouble distinguishing the bubbles from the specimen when looking under the microscope. The presence of bubbles can also keep live organisms from moving freely. Another problem is that large bubbles may lower the viewing resolution.Full Answer >
A diaphragm on a microscope is the piece that enables the user to adjust the amount of light that is focused under the specimen being observed. A diaphragm is typically found on higher-power microscopes versus less expensive or toy models.Full Answer >