The premise of Batman 2: DC Super Heroes is that Lex Luthor has teamed up with the Joker to stop Batman and Superman, with the help of a weapon that creates synthetic Kryptonite. The game features several DC superheroes and villains, even more so than the previous entry.
During the "Man of the Year" awards, for which Lex Luthor and Bruce Wayne are both nominated, several Batman villains, led by the Joker, interrupt the ceremony and the Joker takes the award for himself. Batman joins up with Robin and proceeds to capture and imprison the villains, but Lex Luthor soon arrives at Arkham Asylum and frees the Joker with his new weapon. Lex Luthor gives the weapon to the Joker and the Joker uses it to release all of the Arkham Asylum inmates.
The main goal of the game is to stop both Lex Luthor and the Joker from achieving their goals while also capturing all of the escaped criminals from Arkham Asylum. The game gives the user control over Batman and Superman, but there is also the option to play as several other heroes and villains and even as alternate versions of them. The game also has several collectibles and items that are only retrievable when exploring the same levels with different characters.