Generally speaking, glycerin suppositories take between 15 and 60 minutes to work, according to Drugs.com. While waiting for the suppository to work, it is recommended that individuals limit movement or lie down completely until they feel the urge to have a bowel movement.
People should not use a suppository more than once every 24 hours, according to Drugs.com. The suppository should not be used with other laxatives, as their prolonged use has been linked to damage of the muscles, nerves and tissues of the intestines. Individuals who don't have a bowel movement after one hour has passed are advised to call the doctor.