While as of 2015 the deficiency of any one vitamin is not a known cause of fasciculations, or benign muscle twitches, a lack of the mineral magnesium is a cause, according to Popular Science. A lack of sleep, the over-consumption of certain stimulants and over-exercising are other causes of fasciculations.
Fasciculations result from the irritation of the nerve fibers, most commonly experienced in the eyelids and limbs, explains Popular Science. They are common and occur in over 90 percent of persons at some point in their lives. A less common and more serious cause of muscle twitches is fibrillation, which indicates that the muscle fibers surrounding the twitching muscle have lost their nerve supply, something that occurs in certain conditions, such as Lou Gehrig's Disease.