Dave Kerpen, CEO of the tech startup Likeable Local, recommends asking the question "What will my work contribute to the company's mission?" as the best question to ask at an interview. This question indicates that the candidate is looking for a job to believe in and a compatible company culture.
It is advisable for candidates to use their opportunity to ask questions of hiring managers to reinforce their interest in the company and reassert themselves as a good fit with the workplace. If possible, reference specific details about the company in the question. These details can be gleaned from research into the company's business practices or stated mission conducted prior to the interview.