Hours vary by location, but most of Fifth Third Bank's branches open at 9 a.m., Monday through Friday. Two branches in downtown Cincinnati and one in downtown Dayton open daily at 8 a.m. Branches within retail stores typically open at 10 a.m. and are open seven days a week.
As of July 2015, Fifth Third Bank operates 1,300 branch locations, including 100 within grocery stores that are open seven days a week. Branches set their own hours, but regular branches usually close at 5 p.m., Monday through Thursday and 6 p.m. on Friday. Some branches are open on Saturday, typically for a half day, from 9 a.m. to 1 p.m.