Spider-Man has had many enemies in his comic book and movie career, such as Doctor Octopus and the Green Goblin. The complete list is often referred to as the "rogue's gallery," according to Comic Vine.
Comic Vine notes that one of Spider-Man's primary antagonists is Norman Osborn, otherwise known as the Green Goblin. In time, Norman's son Harry takes over the role.
Doctor Octopus is a nuclear physicist who has his prized invention, a set of mechanical arms, fused to him in an explosion caused by a radiation leak. After this incident led to a criminal turn, Doctor Octopus started the Sinister Six, a supervillain group devoted to destroying Spider-Man. The other original members of the Sinister Six include Electro, Kraven the Hunter, Mysterio, Sandman and Vulture. In time, Kraven the Hunter is replaced by the Hobgoblin.
Venom, a dark and violent mirror of Spider-Man, is another regular foe. He was born when Spider-Man detached an evil, alien-symbiote from his suit, which then latched on to the jealous Eddie Brock.
The Kingpin is a criminal overlord who regularly tangles with all sorts of Marvel heroes, including Spider-Man. Other prominent recurring Spider-Man villains include: Lizard, Carnage, Scorpion, Rhino, Chameleon, Carrion, Jackal, Morbius and Jack O'Lantern.