It has wheelchair access and is used by V/Line's three return passenger trains to Melbourne Southern Cross on weekdays and Saturdays, with two return workings on Sundays. Warrnambool is also a major connecting point for V/Line road coach services to Hamilton, Mount Gambier (SA), Portland, Port Fairy, Ballarat, a new thrice-weekly coach to Ararat via Halls Gap and on Sundays, one remaining return working to Geelong.
There is also a five nights a week freight train conveying containers between Melbourne Freight Terminal and West Vic siding just past Warrnambool. It is operated by El Zorro, who took over after the withdrawal of Pacific National in 2008.
The line once continued west to Port Fairy and is not being converted into a rail trail. Between 1993 and 2004 the private company West Coast Railway operated passenger trains on the line. After their demise V/Line have been operating the passenger service.
On July 14, 2008 the freight shed siding at the up side of the passenger platform was booked out of use, the points abolished and the siding removed. On July 24 the line between the 270.468 km mark and Dennington was officially booked out of use.