Trypanothione-dependent enzymes include reductases, peroxidases, glyoxalases and transferases. Trypanothione-disulfide reductase (TryR) was the first trypanothione-dependent enzyme to be discovered (EC 18.104.22.168). It is an NADPH-dependent flavoenzyme that reduces trypanothione disulfide. TryR is essential for survival of these parasites both in vitro and in the human host.
A major function of trypanothione is in the defence against oxidative stress. Here, trypanothione-dependent enzymes such as tryparedoxin peroxidase (TryP) reduce peroxides using electrons donated either directly from trypanothione, or via the redox intermediate tryparedoxin (TryX). Trypanothione-dependent hydrogen peroxide metabolism is particularly important in these organisms because they lack catalase. Since the trypanosomatids also lack an equivalent of thioredoxin reductase, trypanothione reductase is the sole path that electrons can take from NADPH to these antioxidant enzymes.
US Patent Issued to Council of Scientific and Industrial Research on Aug. 31 for "Heterologus Expression of Trypanothione Reductase from Leishmania Donovani in a Prokaryotic System" (Indian Inventors)
Sep 01, 2010; ALEXANDRIA, Va., Sept. 4 -- United States Patent no. 7,785,850, issued on Aug. 31, was assigned to Council of Scientific and...