This article contains information on the 1985-1986 season of Serie B, the second highest football league in Italy.
| P | Team | Pld | W | D | L | GF | GA | GD | Pts | Promotion or relegation |
|---|---|---|---|---|---|---|---|---|---|---|
| 1. | Ascoli | 38 | 17 | 16 | 5 | 56 | 33 | +23 | 50 | Promoted to Serie A |
| 2. | Brescia | 38 | 17 | 13 | 8 | 41 | 28 | +13 | 47 | |
| 3. | Lanerossi Vicenza | 38 | 16 | 14 | 8 | 48 | 33 | +15 | 46 | |
| 4. | Empoli | 38 | 13 | 19 | 6 | 32 | 28 | +4 | 45 | Promoted to Serie A |
| 5. | Triestina | 38 | 15 | 15 | 8 | 40 | 30 | +10 | 44 | |
| 6. | Bologna | 38 | 15 | 11 | 12 | 37 | 29 | +8 | 41 | |
| 7. | Genoa | 38 | 14 | 12 | 12 | 35 | 31 | +4 | 40 | |
| 8. | Cesena | 38 | 12 | 15 | 11 | 42 | 38 | +4 | 39 | |
| 9. | Cremonese | 38 | 10 | 17 | 11 | 35 | 31 | +4 | 37 | |
| 9. | Campobasso | 38 | 9 | 19 | 10 | 30 | 36 | -6 | 37 | |
| 11. | Arezzo | 38 | 9 | 18 | 11 | 37 | 40 | -3 | 36 | |
| 11. | Lazio | 38 | 11 | 14 | 13 | 38 | 42 | -4 | 36 | |
| 11. | Catania | 38 | 12 | 12 | 14 | 30 | 37 | -7 | 36 | |
| 14. | Sambenedettese | 38 | 10 | 15 | 13 | 26 | 26 | +0 | 35 | |
| 14. | Cagliari | 38 | 13 | 9 | 16 | 27 | 38 | -11 | 35 | |
| 16. | Palermo | 38 | 7 | 20 | 11 | 27 | 35 | -8 | 34 | Excluded |
| 17. | Pescara | 38 | 10 | 13 | 15 | 33 | 37 | -4 | 33 | |
| 18. | Perugia | 38 | 8 | 16 | 14 | 29 | 39 | -10 | 32 | Relegated to Serie C2 |
| 19. | Catanzaro | 38 | 9 | 12 | 17 | 33 | 45 | -12 | 30 | Relegated to Serie C1 |
| 20. | Monza | 38 | 6 | 14 | 18 | 25 | 45 | -20 | 26 |