• Tony Almeida in the Fox TV Series 24
• Hal Ambler, in The Ambler Warning by Robert Ludlum
• Cate Archer in No One Lives Forever
• Basil Argyros in the series of the same name
• Bishop and Shiela from the PS2 video game Spy Fiction
• Jack Bauer in the Fox TV Series 24
• Modesty Blaise
• James Bond in Ian Fleming's novels and the movie series
• Felix Leiter CIA agent and friend of James Bond in numerous novels and films.
• List of James Bond allies for a complete list of 00-agents and secret agents found throughout the film and novel series
• Hans Kloss/J-23 in Stawka większa niż życie (Stakes Larger Than Life)
• Jason Bourne in Robert Ludlum's novels
• Sydney Bristow in Alias
• Xander Cage (Vin Diesel) in XXX
• Callan in Callan
• Adam Carter in Spooks
• Nick Carter (book series)
• Lemmy Caution, in Peter Cheney's novels
• Jerry Cornelius in Michael Moorcock's novels and short-stories
• John Craig in James Munro's four novels
• Joanna Dark in the Nintendo 64 video game Perfect Dark
• John Drake in Danger Man
• Sam Durell in Edward S. Aarons' novels
• Johnny Fedora in Desmond Cory's novels
• Sam Fisher in Tom Clancy's Splinter Cell videogames and books
• Felicity Sarah Flint in the webcomics Basil Flint, P.I. and Felicity Flint, Agent from H.A.R.M.
• Wyman Ford, from several novels by Douglas Preston
• Nick Fury, Black Widow, and Elektra Natchios in the Marvel Comics Universe
• Elim Garak from Star Trek: Deep Space Nine
• Luther Sloan from Star Trek: Deep Space Nine
• Carl Hamilton, Swedish fictional secret agent from the books of Jan Guillou.
• Mylene Hoffman, from the anime 009-1
• Ethan Hunt in Mission: Impossible films
• Michael 'Desolation' Jones from Desolation Jones
• Paul Janson in The Janson Directive
• Brianna Kelleher in Access Denied
• Leon S Kennedy, from Resident Evil 4
• Alec Leamas, in John le Carré's The Spy Who Came in from the Cold
• The Man from U.N.C.L.E. 1960s TV show; Napoleon Solo and Illya Kuryakin
• Matt in Death Note
• Stephen Metcalfe in "The Tristan Betrayal" by Robert Ludlum
• Cammie Morgan, from I'd Tell You I Love You, But Then I'd Have to Kill You
• Nikita
• Elihu 'Sam' Nivens in The Puppet Masters
• Number Six in The Prisoner
• Harry Palmer
• Emily Pollifax in Dorothy Gilman's novels
• Kim Possible
• Quiller
• Tom Quinn (Matthew Macfadyen) in Spooks
• Mitch Rapp
• Alex Rider
• Jack Ryan, in The Hunt for Red October and Patriot Games
• Sean Ryan, ex-IRA member who features in series of novels by Brian Cleeve
• Sam, Clover and Alex from Totally Spies
• Sam in Spynet
• George Smiley from John le Carré's novels
• Solid Snake, Naked Snake and Raiden in the Metal Gear series of videogames
• John Steed, Cathy Gale Emma Peel and Tara King in The Avengers
• John Steed' Mike Gambit and Purdy in The New Avengers
• John Stone, agent of S.T.O.R.M. in Wildstorm's comic Planetary
• Darrius Stone (Ice Cube) in XXX: State of the Union
• Simon Templar, The Saint
• Mr. Verloc in Joseph Conrad's The Secret Agent
• Charles Vine in Licensed to Kill (1965 film)/The Second Best Secret Agent in the Whole Wide World, Where the Bullets Fly, Somebody's Stolen Our Russian Spy
• John & Jane Smith, from Mr. and Mrs. Smith
• Harry Tasker in True Lies
• Father Unwin from The Secret Service
• Michael Westen from the USA original series "Burn Notice"
• The Spy in the Team Fortress 2 videogame
• Rouge the Bat is a secret agent for GUN in the Sonic the Hedgehog series.
• Shadow the Hedgehog is a secret agent for GUN in the Sonic the Hedgehog series.

Parodies of secret agents:

• Agent 327
• Johnny English
• Derek Flint from Our Man Flint and In Like Flint
• I Spy
• Austin Powers
• Brandon Scofield in The Matarese Circle and The Matarese Countdown
• Maxwell Smart and Agent 99 from Get Smart, 1960s TV show
• Desmond Simpkins from Carry On Spying
• Kumar Horowitz - A day with moon