The portion of Harbor Boulevard between La Palma Avenue in Anaheim to Whittier Boulevard in La Habra was part of the historical routing of U.S. Route 101, which was at the time the Coast Route from the Mexican Border to Oregon.
Anaheim native Gwen Stefani mentions Harbor Boulevard in the Song Cool.
| Location | Mile | Destinations | Notes |
|---|---|---|---|
| Newport Beach | 0.0 | Newport Boulevard (SR 55) | South end of Harbor Boulevard |
| Costa Mesa | 3.3 | Interchange | |
| Fountain Valley | 6.0 | Edinger Avenue | |
| Garden Grove | 8.8 | Interchange | |
| Anaheim | 12.2 | Interchange | |
| 13.5 | Lincoln Avenue | Former SR 214 | |
| 14.9 | Interchange | ||
| 20.8 | Whittier Boulevard (SR 72) | ||
| Fullerton, La Habra | Hacienda Boulevard (SR 39) | ||
| Rowland Heights | 23.2 | Fullerton Road | North end of Harbor Boulevard |