Giemsa studied pharmacy and mineralogy at the University of Leipzig, and chemistrry and bacteriology at the University of Berlin. Between 1895 and 1898 he worked as a pharmacist in German East Africa. He was an early assistant to Bernhard Nocht at the Institut für Tropenmedizin in Hamburg, where in 1900 he became head of the Department of Chemistry.
In 1904 Giemsa published an essay on the staining procedure for flagellates, blood cells, and bacteria. Giemsa improved the Romanowsky stain (Eosin Y and Methylene Blue) by stabilizing this dye solution with glycerol. This allowed for reproducible staining of cells for microscopy purposes. This method is still used in laboratories today.