He entered the University of Rome in 1869, where he studied mathematics with Giuseppe Battaglini, Eugenio Beltrami, and Luigi Cremona, obtaining his PhD. in 1875.
In 1885 he published a paper where he defined a certain subgroup of a finite group. This subgroup, now known as the Frattini subgroup, is the subgroup Φ(G) generated by all the non-generators of the group G. He showed that Φ(G) is nilpotent and, in so doing, developed a method of proof known today as Frattini's argument.