Far-Eastern blot

Far-Eastern blotting is a technique developed in the 1990s by T. Taki and colleagues at the Cellular Technology Institute of Otsuka Pharmaceutical Co., Japan for the analysis of lipids separated by high-performance thin layer chromatography (HPTLC). The lipids are transferred from the HPTLC plate to a PVDF membrane for further analysis, for example by enzymatic or ligand binding assays or mass spectrometry.

Cholesterol, glycerophospholipids and sphingolipids are major constituents of the cell membrane and in certain cases function as second messengers in cell proliferation, apoptosis and cell adhesion in inflammation and tumor metastasis. Far-eastern blotting was established as a method for transferring lipids from an HPTLC plate to a polyvinyledene difluoride (PVDF) membrane within a minute. Applications of this with other methods have been studied. Far-eastern blotting allows for the following techniques:

• Purification of glycosphingolipids and phospholipids.
• Structural analysis of lipids in conjunction with direct mass spectrometry.
• Binding study using various ligands such as antibodies, lectins, bacterium, viruses, and toxins, and
• Enzyme reaction on membranes.

Not only analysis of lipids but also metabolites of drugs and natural compounds from plants, and environmental hormones are possible by this method.

References

See also

• Western blot
• Southern blot
• Northern blot