Born Elizabeth Henrietta Campbell, she was the youngest daughter of John Campbell of Airds, Scotland. A distant cousin of Macquarie's she first met him at the age of 26 when he was an army officer. They were married three years later in 1807. Shortly after, in 1809, he was appointed to the governorship of New South Wales and she followed him. She is said to have taken a particular interest in the welfare of women convicts and indigenous people as well as helping pioneer hay making in the colony. At the end of his term, she returned with him to Scotland. The Macquaries had two children, a girl Jane who died in infancy and a boy Lachlan.