This sequel begins a few moments after the previous film ends: Count Dracula has just been killed by Professor Van Helsing (Edward Van Sloan). Van Helsing is taken by police to Scotland Yard, where he explains that he indeed did kill Count Dracula, but because he was already dead for over 500 years, it can't be considered murder. Van Helsing, instead of hiring a lawyer, enlists the aid of a psychiatrist who was once one of his star students. Meanwhile, Dracula's daughter, Countess Marya Zaleska (Gloria Holden), with the aid of her companion, Sandor (Irving Pichel), steals Dracula’s body from Scotland Yard and burns it so she can be a normal mortal. However, her eternal thirst for human blood can't be quenched; the Countess continues to kill and sleep in her coffin during daylight hours. After a chance meeting with the same doctor who is defending Van Helsing, the Countess asks the psychiatrist to help cure her vampirism, without telling the man that she is actually a vampire. As she realizes a cure isn't possible — and the doctor discovers the truth about her condition — the Countess lures him to Transylvania by kidnapping the woman he loves. In the end, she dies when her servant shoots her with a crossbow, as revenge for her breaking her promise to make him immortal.
Bela Lugosi was originally scheduled to reprise his role of Dracula for this sequel - and even appeared in several publicity stills - but for unknown reasons, possibly linked to James Whale's departure, the production went ahead without its original star. However, Lugosi had a pay-or-play contract, and thus received one of his largest salaries despite having not even appeared in the film.
A scene with lesbian implications between the countess and a young female victim is featured in the documentary The Celluloid Closet (1995).