The Abydos King List lists the second pharaoh as Teti, the Turin Canon lists Iteti, while Manetho lists Athothis. Some scholars, however, debate whether the first pharaoh, Menes or Narmer, and Hor-Aha might have been different rulers. If they were separate rulers, this would make Djer the third pharaoh in the dynasty. A mummified wrist of Djer or his wife was discovered, but has been lost.
The inscriptions, on ivory and wood, are in a very early form of hieroglyphs, hindering complete translation, but a wooden seal print at Saqqarah seems to depict the early Old Kingdom practice of human sacrifice. An ivory tablet from Abydos mentions that Djer visited Buto and Sais in the Nile Delta. One of his regnal years on the Cairo Stone was named "Year of smiting the land of Setjet", which often is speculated to be Sinai or beyond.
Similarly to his predecessor, Hor-Aha, Djer was buried in the holy place, Abydos. His tomb contains the remains of 300 retainers who were buried with him. Close to his tomb is that of Merneith, who was his daughter. She also was buried with the honors given to a ruling pharaoh, including many retainers. She was the wife of the next pharaoh, Djet, and mother of Den, for whom she may have served as regent, if not as pharaoh between the two. From the eighteenth dynasty, the tomb of Hor-Aha was revered as the tomb of Osiris and this first dynasty burial complex including the tomb of Djer, was very important in the Egyptian religious tradition.
Manetho indicates that the first dynasty ruled from Memphis, and a wife of Djer named, Herneith, is buried at Saqqarah. Manetho also claimed that Athothes, who is sometimes identified as Djer, had written a treatise on anatomy that still existed in his own day, over two millennia later.