• Desdemona, a fictional character in a tale found in the Hecatommithi (1565) by Cinthio (Giovanni Battista Giraldi)
• Desdemona (Othello), a fictional character in William Shakespeare's play Othello (1603)
• Desdemona (Otello), a soprano role in Giuseppe Verdi's opera Otello (1887)
• Desdemona, a rôle from Shakespeare's Othello in the film O, renamed Desi Brable, played by Julia Stiles (1999/2001)
• Desdemona Stephanides, a character in the book Middlesex by author Jeffrey Eugenides
• Desdemona, a character in the book Eleven Hours by author Paullina Simons
• Desdemona, a character in the book Trust Me by author Jayne Ann Krentz
• Desdemona, a character recreated as Kynaston, played by Billy Crudup the film Stage Beauty
• Desdemona, a character in the book Vurt by author Jeff Noon
• Desdemona, a character in the book The Midnighters by the author Scott Westerfeld
• Desdemona, a character in a Jimmy Buffett novel Joe Merchant
• Desdemona, a character in the animated TV series Gargoyles
• Desdemona, a cat character in the comic strip Mutt and Jeff

Desdemona, in song, may refer to:

• Desdemona (band), an Italian power metal band
• Desdemona (Allman Brothers song), a song by The Allman Brothers
• Desdemona (Marc Bolan song), a song first recorded by John's Children
• Desdemona, the subject of the song Desdemona's Building a Rocket Ship on the Banana Wind album June 4, 1996 (Jimmy Buffett song) which refers to the character Desdemona and events which take place in the Jimmy Buffett novel Joe Merchant
• Desdemona (Eddie Murphy song), a hit single sung by Eddie Murphy (Motown, 1993) (music)
• Desdemona (Billy Merson song), early film, music hall funny song sung by Billy Merson in 1927

Desdemona, as a place, may refer to:

• Desdemona (moon), a moon of Uranus
• 666 Desdemona, an asteroid
• Desdemona, Texas

Desdemona, as a thing, may refer to:

• Desdemona, a typeface included with early versions of Microsoft software, such as Office 4.3
• Desdemona Music Festival, music festival in Cincinnati, Ohio