Due to increasing concerns over speeds and reconstruction of the West Banking (NASCAR Turns 1 and 2) after the 2004 season, Daytona made two changes for 2005. First, the premier AMA Superbike class race was changed from 200 miles (322 km) to 100 kilometers (62 miles), and the Formula Xtreme class was promoted to the Daytona 200. Second, the track configuration was changed such that the motorcycles would run through a short link after passing the Pedro Rodriguez hairpin, then run across the International Horseshoe anticlockwise, and then pass through the infield, rejoining the track on the Superstretch. This eliminated a half mile from the track, eliminated the west banking segment of the course, and thus increased the number of laps required for the race. For the 2009 Daytona 200, Superbikes will again race the premier event; the first change to the racing series following the sale of the AMA Pro Racing series to the Daytona Motorsports Group on March 7, 2008.
The race has been one of the toughest in American motorcycling because of its endurance-like qualities of pit stops for tires and fuel, and safety car periods, and nine FIM world champions, including seven 500cc/MotoGP World Champions -- six Americans and one Italian -- have won the race. Of recent American world champions, only Kenny Roberts, Jr. did not win the Daytona 200. Finnish and Venezuelan FIM world champions in smaller classes have also won the 200.
Scott Russell and Miguel Duhamel are tied for most Daytona 200 wins at five each. It should be noted that Russell, known by the nickname "Mr. Daytona" because of his achievements at the famed track, won all his Daytona races in the Superbike class (750-1000cc). Duhamel's fifth victory came in the new-for-2005 class, Forumula Xtreme (600cc).
Steve Rapp's 2007 victory was the first win for Kawasaki since 1995 and the first win for a non-factory rider since John Ashmead won in 1989.
| Year | Rider | Country | Manufacturer | Class | Course |
| 2008 | Chaz Davies* | Kawasaki | Formula Xtreme | 2.95 mile Daytona Speedway/Infield Course | |
| 2007 | Steve Rapp | Kawasaki | Formula Xtreme | 2.95 mile Daytona Speedway/Infield Course | |
| 2006 | Jake Zemke | Honda | Formula Xtreme | 2.95 mile Daytona Speedway/Infield Course | |
| 2005 | Miguel Duhamel | Honda | Formula Xtreme | 2.95 mile Daytona Speedway/Infield Course | |
| 2004 | Mat Mladin | Suzuki | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 2003 | Miguel Duhamel | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 2002 | Nicky Hayden | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 2001 | Mat Mladin | Suzuki | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 2000 | Mat Mladin | Suzuki | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1999 | Miguel Duhamel | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1998 | Scott Russell | Yamaha | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1997 | Scott Russell | Yamaha | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1996 | Miguel Duhamel | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1995 | Scott Russell | Kawasaki | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1994 | Scott Russell | Kawasaki | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1993 | Eddie Lawson | Yamaha | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1992 | Scott Russell | Kawasaki | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1991 | Miguel Duhamel | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1990 | David Sadowski | Yamaha | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1989 | John Ashmead | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1988 | Kevin Schwantz | Suzuki | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1987 | Wayne Rainey | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1986 | Eddie Lawson | Yamaha | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1985 | Freddie Spencer | Honda | Superbike | 3.56 mile Daytona Speedway/Infield Course | |
| 1984 | Kenny Roberts | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1983 | Kenny Roberts | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1982 | Graeme Crosby | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1981 | Dale Singleton | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1980 | Patrick Pons | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1979 | Dale Singleton | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1978 | Kenny Roberts | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1977 | Steve Baker | Yamaha | Formula 1 | 3.87 mile Daytona Speedway/Infield Course | |
| 1976 | Johnny Cecotto | Yamaha | -- | 3.87 mile Daytona Speedway/Infield Course | |
| 1975 | Gene Romero | Yamaha | -- | 3.84 mile Daytona Speedway/Infield Course | |
| 1974 | Giacomo Agostini | Yamaha | -- | 3.84 mile Daytona Speedway/Infield Course | |
| 1973 | Jarno Saarinen | Yamaha | -- | 3.84 mile Daytona Speedway/Infield Course | |
| 1972 | Don Emde | Yamaha | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1971 | Dick Mann | BSA | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1970 | Dick Mann | Honda | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1969 | Cal Rayborn | Harley-Davidson | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1968 | Cal Rayborn | Harley-Davidson | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1967 | Gary Nixon | Triumph | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1966 | Buddy Elmore | Triumph | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1965 | Roger Reiman | Harley-Davidson | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1964 | Roger Reiman | Harley-Davidson | -- | 3.81 mile Daytona Speedway/Infield Course | |
| 1963 | Ralph White | Harley-Davidson | -- | 2 mile Daytona Speedway/Infield Course | |
| 1962 | Don Burnett | Triumph | -- | 2 mile Daytona Speedway/Infield Course | |
| 1961 | Roger Reiman | Harley-Davidson | -- | 2 mile Daytona Speedway/Infield Course | |
| 1960 | Brad Andres | Harley-Davidson | -- | 4.1 mile Daytona Beach Course | |
| 1959 | Brad Andres | Harley-Davidson | -- | 4.1 mile Daytona Beach Course | |
| 1958 | Joe Leonard | Harley-Davidson | -- | 4.1 mile Daytona Beach Course | |
| 1957 | Joe Leonard | Harley-Davidson | -- | 4.1 mile Daytona Beach Course | |
| 1956 | John Gibson | Harley-Davidson | -- | 4.1 mile Daytona Beach Course | |
| 1955 | Brad Andres | Harley-Davidson | -- | 4.1 mile Daytona Beach Course | |
| 1954 | Bobby Hill | BSA | -- | 4.1 mile Daytona Beach Course | |
| 1953 | Paul Goldsmith | Harley-Davidson | -- | 4.1 mile Daytona Beach Course | |
| 1952 | Dick Klamfoth | Norton | -- | 4.1 mile Daytona Beach Course | |
| 1951 | Dick Klamfoth | Norton | -- | 4.1 mile Daytona Beach Course | |
| 1950 | Billy Mathews | Norton | -- | 4.1 mile Daytona Beach Course | |
| 1949 | Dick Klamfoth | Norton | -- | 4.1 mile Daytona Beach Course | |
| 1948 | Floyd Emde | Indian | -- | 4.1 mile Daytona Beach Course | |
| 1947 | John Spiegelhoff | Indian | -- | 3.2 mile Daytona Beach Course | |
| 1941 | Billy Mathews | Norton | -- | 3.2 mile Daytona Beach Course | |
| 1940 | Babe Tancrede | Harley-Davidson | -- | 3.2 mile Daytona Beach Course | |
| 1939 | Ben Campanale | Harley-Davidson | -- | 3.2 mile Daytona Beach Course | |
| 1938 | Ben Campanale | Harley-Davidson | -- | 3.2 mile Daytona Beach Course | |
| 1937 | Ed Kretz | Indian | -- | 3.2 mile Daytona Beach Course |