Nikolaus was a son of Wilhelm, Duke of Nassau and his second wife, Pauline of Württemberg. He was also a younger half-brother of Adolphe, last duke of Nassau, and later Grand Duke of Luxembourg. Natalia was a daughter of Russian novelist Alexander Pushkin and his wife Natalya Goncharova, and therefore, through her father, is a descendant of Peter the Great's African protégé, Abram Petrovich Gannibal as well as the Cossac leader Prince Petro Doroshenko. Natalia was created Countess of Merenberg as she was not allowed to use her husband's titles and rank.
Their surviving children were:
The title is not in current use, as the persons entitled to use it are all deceased, some decades ago.
When Duke Nikolaus Wilhelm died in 1905, his nephew Grand Duke William IV of Luxembourg (or Guillaume IV) became the last surviving agnate of the House of Nassau. If Nikolaus Wilhelm's children had been deemed dynastic, then his son Georg Nickolaus Count of Merenberg, would have succeeded as Head of the House of Nassau upon William IV's death. Georg Nickolaus would have thus become the reigning Grand Duke of Luxembourg.
However, his morganatic birth was deemed unsurmountable. In 1907, as Head of the House of Nassau, William IV, declared the Counts of Merenberg non-dynastic in regard to the Nassau Family Pact. This excluded the family from succession to Luxembourg's throne.
Instead, Williams IV's daughter Marie-Adélaïde succeeded her father to become Luxembourg's first female monarch in 1912. She was in turn succeeded by her sister Charlotte, whose descendants reign to the present day.
The heads of the house of Merenberg after 1912 were:
It is unclear what would have happened to the succession after 1965, had they inherited the Nassau patrimony.