Glover was an aspiring actor when Vernon Reid drafted him into Living Colour, reportedly after seeing Glover singing "Happy Birthday" at a friend's party. He appeared as Pvt. Francis in Oliver Stone's classic Vietnam war film, Platoon, and starred in a short lived television series called "Signs of Life".
After Living Colour's split in 1995, he started a solo career as Reverend Daddy Love and is lead vocalist for his band VICE with Mike Ciro. He has also hosted various shows on VH1.
Living Colour reunited in 2000 at CBGB's while playing with Will Calhoun and Doug Wimbish's live drum 'n' bass duo, HEADFAKE, Vernon Reid joined them three songs into the HEADFAKE setlist and Living Colour was back again. Glover is once again singing lead vocal with them.
Living Colour released Collideøscope in October 2003.
In August 2006, Glover began co-headlining a national tour of Jesus Christ Superstar as Judas, opposite Ted Neeley. Carl Anderson, famous for portraying Judas since 1971 alongside Neeley, was set to reprise the role but had died of leukemia in 2004. The tour is slated to run through 2009, but now is reportedly set to run indefinately depending upon audience and critical reception. Glover told Neeley that when he was a child, seeing the movie version of the show was what made him decide to be an entertainer. 
In 2007 Living Colour re-recorded "Cult of Personality" for the popular game Guitar Hero 3.