Ascot Vale is a suburb in Melbourne, Victoria, Australia. Its Local Government Area is the City of Moonee Valley.
Tram route 57 runs along Epsom Road, Union Road and Maribyrnong Road, route 59 runs along Mount Alexander Road, and route 82 runs along Maribyrnong Road.
A major landmark in the suburb are the Royal Melbourne Showgrounds, only open during special events, such as the annual Royal Melbourne Show. The Showgrounds are adjacent to the Flemington Racecourse which are the in neighbouring Flemington.
Ascot Vale has Ascot Vale Primary School, Ascot Vale West Primary School, St Mary's Catholic Primary School and the arts program of Western Autistic School