At the age of 18, Anita entered the Miss Hong Kong 1990 pageant. She was a heavy favorite to win the crown from the semifinal to final. During the semifinals on August 5, 1990, she won the Miss Photogenic award. She had a semifinal score of 485, placing 3rd overall. The finals were held on August 21, 1990. She eventually beat out another big favorite, Helen Yung who was 1st runner up and the winner of 2 other awards. Anita later represented Hong Kong at the Miss Chinese International 1991 pageant, held on February 2, 1991. She took 1st runner up for Hong Kong. She also competed at the Miss Universe 1991 pageant in Las Vegas, Nevada. She failed to make the top 10 and was placed 60th. The pageant was won by Miss Mexico, Lupita Jones.
Anita Yuen is best known for her role as the female lead in Derek Yee's 1993 tear-jerker C'est la vie, mon chéri (1993). She is also well remembered in her cross-gender comic role opposite Leslie Cheung in He's a Woman, She's a Man (1994). The two roles won for her the Hong Kong Academy Best Actress awards. She has also acted in a number of television serials, notably in Taiwan.
Her boyfriend is 36 year old Hong Kong actor and singer Julian Cheung. In May 2006, Yuen announced that she was 3 months pregnant with their first child. Their son, Morton Cheung, was born on November 12, 2006. On June 23, 2007, Anita was a guest on TVB's Be My Guest with host Stephen Chan revealing that she and Julian were secretly married in the United States, San Francisco, back in 2001.