Diseases caused by Acanthamoeba include amoebic keratitis and encephalitis. The latter is caused by Acanthamoeba entering cuts and spreading to the central nervous system. The former is a rare disease where amoebae invade the cornea of the eye. In the United States, it is nearly always associated with contact lens use, as Acanthamoeba can survive in the space between the lens and the eye. However, elsewhere in the world, many cases of Acanthamoeba present in non-contact lens wearers. For this reason, contact lenses must be properly disinfected before wearing, and should be removed when swimming or surfing.
To detect Acanthamoeba on a contact lens in a laboratory, a sheep blood agar plate with a layer (a lawn) of E. coli is made. Part of the contact lens is placed on the agar plate. If Acanthamoeba are present, they will ingest the bacteria, leaving a clear patch on the plate around the area of the lens. Polymerase chain reaction can also be used to confirm a diagnosis of Acanthamoeba keratitis, especially when contact lenses are not involved.
Acanthamoeba granulomatous encephalitis is an opportunistic protozoan pathogen that rarely causes disease in humans. Approximately 400 cases have been reported worldwide with a survival rate of only two to three percent. Infection usually occurs in patients with an immunodeficiency, diabetes, malignancies, malnutrition, systemic lupus erythematosus, or alcoholism. The parasite's portal of entry is via lesions in the skin or the upper respiratory tract or via inhalation of airborne cysts. The parasite then spreads hematogenously into the central nervous system. Acanthamoeba crosses the blood brain barrier by means that are not yet understood. Subsequent invasion of the connective tissue and induction of pro-inflammatory responses leads to neuronal damage which can be fatal within days. A post-mortem biopsy reveals severe oedema and hemorrhagic necrosis. A patient that has contracted this illness usually displays subacute symptoms including altered mental status, headaches, fever, neck stiffness, seizures, focal neurological signs such as cranial nerve palsies and coma all leading to death within one week to several months. Due to the rarity of this parasite and our lack of knowledge there are currently no good diagnoses or treatments for Acanthamoeba.
Infection usually mimics that of bacterial leptomeningitis, tuberculous meningitis, or viral encephalitis. The misdiagnosis often leads to erroneous treatment that is ineffective. In the case that Acanthamoeba is diagnosed correctly, the current treatments such as amphotericin-B, rifampicin, trimethroprim-sulfamethoxazole, ketokonazole, fluconazole, sulfadiazine, albendazole are only tentatively successful. Correct and timely diagnosis as well as improved treatment methods as well as understanding of the parasite are important factors in improving the outcome of infection by Acanthamoeba.
This species is able to lyse bacteria and produce a wide range of enzymes such as cellulases or chitinases and probably contributes to the break down of organic matter in soil, contributing to the microbial loop.
Acanthamoeba sp. contain diverse bacterial endosymbionts which are similar to human pathogens. Because of this they are considered to be potential emerging human pathogens. The exact nature of these symbionts and the benefit they represent for the amoebal host still have to be clarified.
Granulomatous inflammation in acanthamoeba keratitis: An immunohistochemical study of five cases and review of literature.(Original Article)
Oct 01, 2005; Byline: G. Vemuganti, G. Pasricha, S. Sharma, P. Garg Purpose: Acanthamoeba keratitis usually presents as a necrotizing...
A study of the spectrum of Acanthamoeba keratitis: A three-year study at a tertiary eye care referral center in South India.(Clinical report)
Jan 01, 2007; Byline: Jayahar. Bharathi, M. Srinivasan, R. Ramakrishnan, R. Meenakshi, S. Padmavathy, Prajna. Lalitha Purpose: To determine the...