Starting at the Prime Meridian and heading eastwards, the parallel 40° north passes through:
| Country, territory or sea | Notes |
|---|---|
| Mediterranean Sea | |
| Island of Minorca | |
| Mediterranean Sea | |
| Island of Sardinia | |
| Mediterranean Sea | Tyrrhenian Sea |
| Mediterranean Sea | Gulf of Taranto |
| Mediterranean Sea | Strait of Otranto |
| Aegean Sea | Including the Kassandra and Sithonia peninsulas, and the island of Lemnos () |
| Including Nagorno-Karabakh | |
| Caspian Sea | |
| Sokh exclave surrounded by | |
| Shakhimardan exclave surrounded by | |
| Yellow Sea | Liaodong Bay |
| Liaodong Peninsula | |
| Sea of Japan | |
| Island of Honshū | |
| Pacific Ocean | |
| California Nevada Utah Colorado Nebraska / Kansas border Missouri Illinois Indiana Ohio West Virginia Pennsylvania New Jersey | |
| Atlantic Ocean | |
The parallel 40° north formed the original northern boundary of the British Colony of Maryland. A subsequent royal grant gave the Colony of Pennsylvania land north of the parallel 39° north. The Mason-Dixon Line was drawn between 1763 and 1767 as the compromise boundary between the overlapping claims of these two colonies.
The parallel 40° north passes through the cities of Philadelphia, Pennsylvania and Columbus, Ohio; as well as northern suburbs of Indianapolis, Indiana and Denver, Colorado. The parallel goes directly through the Port Columbus International Airport, with runway 10L-28R lying immediately north of the line, runway 10R-28L lying slightly south of it, and the line going through the northernmost edges of the terminal.
Baseline Road in Boulder, Colorado traces the parallel 40° north.