trichrome stain

Masson's trichrome stain

Masson's trichrome is a three-color staining protocol used in histology. The recipes evolved from the original Masson's formulation to different specific applications, but all are suited for distinguishing cells from surrounding connective tissue.

Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell nuclei.

The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C:

A common variant is Lillie's trichrome. It is often errorneously called Masson's trichrome. It differs in the dyes used, their concentrations, and the immersion times.

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