After UFO closed, Interfisch's head, Dimitri Hegemann and some investors in the club found the new space in East Berlin. This was advantageous timing, as it was only a few months before Germany unified. The vaults under the Wertheim department store proved to be the perfect location for a club, and Tresor quickly became the place to be in Berlin. Tresor continued to be a popular club to this day, having expanded and reconstructed continuously several times to include an outdoor garden area, and a second "Globus" floor. The concept for the Tresor floor in the basement was specifically hard techno, industrial and acid music while Globus was featuring mainly more mellow house sound. The recordlabel Tresor Records was founded soon after the club first opened, in October 1991. Featured artists on the label include Jeff Mills, Juan Atkins, Robert Hood, Joey Beltram, Pacou, Blake Baxter, Cristian Vogel and many others.
In 2004 the documentary "Tresor Berlin: The Vault & the Electronic Frontier" was released. Directed by Mike Andrawis, it features interviews with Hegemann, Carola Stoiber, and DJs & artists associated with the club & label. The film covers the period from Hegemann's involvement with the Fishladen and UFO clubs in Berlin-Kreuzberg to the final months prior to Tresor's closure.
Tresor closed on the 16th April 2005, after several years prolonged short-term rent. The city sold the land to an investor group to build offices on the Leipziger Straße location. It was open for each night of April 2005, with the final event starting the Saturday night with queues stretching all the way down the road, and still going Monday morning.