TLO can also mean Technology Licensing Office, a branch of many universities.

T. Lo is also the nickname of SmackDown! general manager Theodore Long

T.L.O. also refers to an unnamed high school student involved in the U.S. Supreme Court case New Jersey v. T.L.O.

TLO, or Turun linja-autoilijain osakeyhtiö (Finnish for Turku Bus Operators Ltd) is a group of bus companies and the main operator of the regional public transport in the region of Turku, Finland. TLO was founded in 1954 by over fifty private bus companies. Since then, the number of companies in the group has shrunk to eleven.

On a normal day, 52 of the group's buses are in operation on lines between Turku and its neighbouring cities. The most important destinations of TLO's bus services are:

• Naantali (lines 11, 110, 201)
• Raisio (lines 11, 110, 115, 118, 119, 420, 421, 422)
• the Mylly shopping centre (lines 180, 195, 420, 421)
• Kuninkoja (lines 190, 191, 194, 195)
• Kaarina (lines 11, 110, 111, 116, 192)
• Littoinen (line 110)
• Lieto (line 280)
• Rusko (lines 191, 194, 422)
• Vahto (lines 191, 422)
• Piikkiö (lines 111, 116)
• Paimio (lines 109, 111)
• Masku (lines 118, 119, 422)
• Nousiainen (lines 118, 119)
• Mynämäki (line 118)
• Merimasku, Rymättylä (line 112 from Naantali)
• Lemu, Askainen, Velkua (unnumbered line)