TLC may refer to:

In entertainment:

• TLC (band), an American R&B/hip hop/pop girl group
• TLC (TV channel) or 'The Learning Channel', a cable TV network in the United States and Canada
• TLC (TV series) ('Tender Loving Care'), a British situation comedy airing on BBC2
• Tables, Ladders, and Chairs match, a type of pro wrestling match
• The Last Continent, a Discworld novel by Terry Pratchett

In organizations:

• The Land Conservancy, a not-for-profit, charitable land trust based in British Columbia, Canada
• The Learning Company, an American educational software company
• True and Living Church of Jesus Christ of Saints of the Last Days, an offshoot of The Church of Jesus Christ of Latter-day Saints
• Taxi and Limousine Commission, the New York City government agency that regulates taxicabs and for-hire vehicles

In schools:

• Thayer Learning Center, a controversial boot-camp for teens in rural Missouri
• The Learning Center for the Deaf, a school for the deaf and hard of hearing located in Framingham, Massachusetts
• Robbinsdale Middle School, formerly Technology and Learning Center, located in Robbinsdale, Minnesota

In medicine:

• Total lung capacity, in lung volumes

In other uses:

• Lic. Adolfo López Mateos International Airport, in Toluca, Mexico, whose IATA airport code is TLC
• Thin layer chromatography, a chromatography technique used in chemistry to separate chemical compounds
• Traffic Light Controller
• Trades and Labour Council, an Australian term for a labour council
• Tratado de Libre Comercio, the Spanish name for "Free Trade Agreement"