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Osmium tetroxide is the chemical compound with the formula OsO4. The compound is noteworthy for its many uses, despite the rarity of osmium. It also has a number of interesting properties, one being that the solid is volatile.
Osmium tetroxide exists as a pale yellow-brown crystalline solid with a characteristic acrid odor similar to ozone
. In fact, the element
name osmium is derived from osme
is volatile: it sublimes
at room temperature
. It is soluble in a wide range of organic solvents, and moderately soluble in water, with which it reacts reversibly to form osmic acid (see below). Pure
osmium tetraoxide is probably colourless and it has been suggested that its yellow hue is due to osmium dioxide (OsO2
) impurities although osmium (IV) oxide normally exists as a black powder so this may not be true.
Structure and electron configuration
With a d0
configuration, Os(VIII) is expected to form tetrahedral complexes when bound to four ligands. Tetrahedral structures are seen for the electronically related oxides MnO4−
The osmium of OsO4 has a formal oxidation state of 8+, the highest oxidation state known for a transition metal. The osmium atom has eight valence electrons. If one assumes that two electrons are donated by each of the four oxide ligands, the total electron count for the complex is 16, as also seen for the isoelectronic species permanganate and chromate.
is formed slowly when osmium powder reacts with O2
at 298 K. Reaction of bulk solid requires heating to 670 K.
- Os + 2 O2 → OsO4
Osmium forms several oxofluorides, all of which are very sensitive to moisture.
forms at 77 K in an aqueous solution of HF:
- OsO4 + 2 KrF2 → cis-OsO2F4 + 2 Kr + O2
OsO4 also reacts with F2 to form yellow OsO3F2:
- 2 OsO4 + 2 F2 → 2 OsO3F2 + O2
OsO4 reacts with one equivalent of [Me4N]F at 298 K and 2 equivalents at 253 K:
- OsO4 + [Me4N]F → [Me4N][OsO4F]
- OsO4 + 2 [Me4N]F → [Me4N]2[cis-OsO4F2]
Oxidation of alkenes
catalyzes the cis-dihydroxylation of alkenes by hydrogen peroxide or related sources of oxygen atoms in the presence of water. The reaction that is catalyzed is
- R2C=CR2 + H2O2 → R2C(OH)-C(OH)R2.
In terms of mechanism, OsVIIIO4 adds to alkenes R2C=CR2 to afford cyclic "esters" R4C2O2OsVIO2, which undergo hydrolysis to give the vicinal diol and release a reduced osmium oxide (OsVI):
- Lewis bases such as tertiary amines and pyridines increase the reaction rate. This "ligand-acceleration" arises via the formation of adduct OsO4L, which adds more rapidly to the alkene. If the amine is chiral, then the dihydroxylation can proceed with enantioselectivity (see Sharpless asymmetric dihydroxylation).
Since OsO4 is toxic and expensive, it is used in catalytic amounts. The osmium catalyst is regenerated by oxidizing agents, such as H2O2, N-methylmorpholine N-oxide (NMO, see Upjohn dihydroxylation), and K3Fe(CN)6. These oxidizing reagents do not react with the alkenes on their own. Other sources of osmium tetroxide include potassium osmate(VI) dihydrate (K2OsO4·2H2O) and osmium (III) chloride hydrate (OsCl3.xH2O) which oxidise to osmium (VIII) in the presence of such oxidants.
dissolves in alkaline aqueous solution to give the osmate anion:
- OsO4 + 2 NaOH → Na2[cis-OsO4(OH)2] + O2
OsO4 is a Lewis acid, and when the Lewis bases are amines, the oxides can undergo substitution. Thus with NH3 one obtains the nitrido-oxide:
- OsO4 + NH3 + KOH → K[Os(N)O3] + 2 H2O
anion is isoelectronic and isostructural with OsO4
. Using primary amine tert-BuNH2
one obtains the corresponding imido derivative:
- OsO4 + 4 Me3CNH2 → Os(NCMe3)4 + 4 H2O
OsO4 undergoes "reductive carbonylation" in methanol at 400 K and 200 bar of pressure to produce the triangular cluster Os3(CO)12:
- 3 OsO4 + 24 CO → Os3(CO)12 + 12 CO2
In this reaction osmium changes oxidation state by eight units.
In organic synthesis OsO4
is widely used to oxidise alkenes
to the vicinal diols, adding two hydroxyl
groups at the same side (syn addition
). See reaction and mechanism above. This reaction has been made both catalytic (Upjohn dihydroxylation
) and asymmetric (Sharpless asymmetric dihydroxylation
, named after Nobel Laureate K. Barry Sharpless
Osmium tetroxide is also used in catalytic amount in the Sharpless oxyamination to give vicinal amino-alcohols.
In combination with sodium periodate, OsO4 is used for the oxidative cleavage of alkenes (the Lemieux-Johnson oxidation). Here the periodate serves both to cleave the diol formed by dihydroxylation, and to reoxidize the OsO3 back to OsO4. The net transformation is identical to that produced by ozonolysis. Below an example from the total synthesis of Isosteviol.
is a widely used staining
agent used in transmission electron microscopy
(TEM) to provide contrast to the image. As a lipid
stain, it is also useful in scanning electron microscopy
(SEM) as an alternative to sputter coating
. It embeds a heavy metal directly into cell membranes, creating a high secondary electron
emission without the need for coating the membrane with a layer of metal, which can obscure details of the cell membrane. Additionally, osmium tetroxide is also used for fixing biological samples in conjunction with HgCl2
. Its rapid killing abilities are used to quickly kill specimen like protozoa. Osmium tetroxide is also used as a stain for lipids in optical microscopy. OsO4
also stains the human cornea (see safety considerations
It is also used to stain copolymers
preferentially, the best known example being block copolymers where one phase can be stained so as to show the microstructure
of the material. For example, styrene-butadiene block copolymers have a central polybutadiene
chain with polystyrene end caps. When treated with OsO4
, the butadiene matrix reacts preferentially and so absorbs the oxide. The presence of a heavy metal is sufficient to block the electron beam, so the polystyrene domains are seen clearly in thin films in TEM
can be recycled and stored in the form of osmeth, a golden crystalline solid. Osmeth is OsO4
complexed with hexamine
and does not emit toxic fumes as opposed to pure OsO4
. It can be dissolved in tetrahydrofuran
(THF) and diluted in an aqueous buffer solution
to make a dilute (0.25%) working solution
Osmium ore refining
is an intermediate in osmium ore refining. Osmium residues are reacted with Na2
anions, which, when reacted with chlorine
) gas and heated, form OsO4
. The oxide is dissolved in alcoholic NaOH
anions, which, when reacted with NH4Cl
, forms OsO2
. This is ignited under hydrogen
) gas leaving behind pure osmium (Os).
allowed for the confirmation of the soccer ball model of buckminsterfullerene
, a 60 atom carbon allotrope
. The adduct
, formed from a derivative of OsO4
, was C60
. The adduct broke the fullerene's symmetry allowing for crystallization and confirmation of the structure of C60
by x-ray crystallography
is highly poisonous, even at low exposure levels, and must be handled with appropriate precautions. In particular, inhalation at concentrations well below those at which a smell can be perceived can lead to pulmonary edema
, and subsequent death. Noticeable symptoms can take hours to appear after exposure.
also stains the human cornea
, which can lead to blindness if proper safety precautions are not observed.
On the 6th of April 2004 British intelligence sources believed they had foiled a plot to detonate a bomb involving OsO4. Experts interviewed by New Scientist affirmed osmium tetroxide's toxicity, though some highlighted the difficulties of using it in a weapon: osmium tetroxide is very expensive. The osmium tetroxide may be destroyed by the blast; what remaining toxic fumes may also be dispersed by the blast as well.
- Cotton, S. A. "Chemistry of Precious Metals," Chapman and Hall (London): 1997. ISBN 0-7514-0413-6.
- D. J. Berrisford, C. Bolm and K. B. Sharpless (1995). "Ligand-Accelerated Catalysis". Angewandte Chemie International Edition in English 34 (10): 1059–1070.